Journal: BMB Reports

Article Title: Curcumin ameliorates TNF-α-induced ICAM-1 expression and subsequent THP-1 adhesiveness via the induction of heme oxygenase-1 in the HaCaT cells

doi: 10.5483/BMBRep.2013.46.8.014

Figure Lengend Snippet: HO-1 induction mediates the inhibitory effect of curcumin on TNF-α-induced monocyte adhesion in HaCaT cells. (A) HaCaT cells were incubated with 20 μM curcumin for 6 h in the absence or presence of SnPP, and then exposed to TNF-α (10 ng/ml) for 12 h. HaCaT cells were co-cultured with calcein-AM-labeled THP-1 monocytes for 1 h. The calcein-AM fluorescent intensity was measured by an ELISA plate reader. (B) HaCaT cells transfected with control or HO-1 siRNA were incubated with 20 μM curcumin for 6 h, and stimulated with TNF-α for 12 h. HaCaT cells were co-cultured with calcein-AM-labeled THP-1 monocytes for 1 h. The calcein- AM fluorescent intensity was measured by an ELISA plate reader. Results are means ± SD. Statistical significance: *** P ＜0.001 compared to the TNF-α alone, ## P ＜ 0.01 compared to the TNF-α and curcumin. (C) Microphotographs were obtained using fluorescence microscopy (original magnification, ×40).

Article Snippet: For the adhesion quantification, the fluorescent intensity of each well was measured at λ = 485 nm excitation and λ = 538 nm emission by a Fluoroskan ELISA plate reader (Ani Labsystems Ltd. Oy.

Techniques: Incubation, Cell Culture, Labeling, Enzyme-linked Immunosorbent Assay, Transfection, Control, Fluorescence, Microscopy

Journal: BMB Reports

Article Title: Curcumin ameliorates TNF-α-induced ICAM-1 expression and subsequent THP-1 adhesiveness via the induction of heme oxygenase-1 in the HaCaT cells

doi: 10.5483/BMBRep.2013.46.8.014

Figure Lengend Snippet: siRNA knockdown of Nrf2 reverses the inhibitory effects of curcumin on TNF-α-induced ICAM-1 expression and monocyte adhesion in HaCaT cells. (A) HaCaT cells transfected with control or Nrf2 siRNA were incubated with 20 μM curcumin for 6 h, and stimulated with TNF-α for 1 h (for RNA) and for 12 h (for protein). Total RNA and protein were analyzed by RT-PCR (upper panel) and immunoblotting (bottom panel). (B) HaCaT cells transfected with control or Nrf2 siRNA were incubated with 20 μM curcumin for 6 h, and stimulated with TNF-α for 12 h. HaCaT cells were co-cultured with calcein-AM-labeled THP-1 monocytes for 1 h. The calcein-AM fluorescent intensity was measured by an ELISA plate reader. Results are means ± SD. Statistical significance: *** P ＜ 0.001 compared to the TNF-α alone, ## P ＜ 0.01 compared to the TNF-α and curcumin.

Article Snippet: For the adhesion quantification, the fluorescent intensity of each well was measured at λ = 485 nm excitation and λ = 538 nm emission by a Fluoroskan ELISA plate reader (Ani Labsystems Ltd. Oy.

Techniques: Knockdown, Expressing, Transfection, Control, Incubation, Reverse Transcription Polymerase Chain Reaction, Western Blot, Cell Culture, Labeling, Enzyme-linked Immunosorbent Assay